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200 L of MSSS or 1 mL of sample solution was placed in a centrifuge tube (15 mL), and 0.5 mol/L sodium bicarbonate solution (pH=9.0) 1 mL and 1% DNFB derivatization solution (2,4-dinitrofluorobenzene, abbreviated as DNFB, 1 mL DNFB, acetonitrile to 100 mL) 1 mL, was mixed by vortexing, and then put in a water bath at 60 C for 10 min, and then cooled

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The companies always end up winning lots of money, and the farmers, they stay always the same, Federico Langarica said