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In Vivo Subchronic Inhalation Testing The in vivo animal toxicology portion of Project MIX consisted of exposing male and female Sprague-Dawley rats, nose only, to either fresh air (sham) or diluted mainstream smoke from the Project MIX control and test cigarettes for 90 d, followed by a 42-d postexposure period, after which time the animals were sacrificed and histopathology studies were completed [18]