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Guo et al., DNA Sequencing Development of CVS diseases is associated with up-regulation of miR-206 in smokers compared to non-smokers (Vrijens et al., In utero exposure to maternal smoking is also associated with DNA methylation changes and reduced neuronal development using DNA Methylation and Gene Expression Arrays (Chatterton et al., In silico Models for the CVS Hemodynamics Models Numerous hemodynamics models have been developed targeting at different spatial scales of the CVS, ranging from a large vasculature with thousands of vessels (Muller et al., Calcium Signaling Models In silico models have been proposed for the effects of WSS on ion channel flux in ECs (Comerford et al., 2+ ) influx through intracellular store-operated Ca 2+ channels that are key to activating NO-producing eNOS (Andrews et al., 2+ micro-domains formed in sub-plasma membrane regions known as caveolae (Cohen et al., 2+ mediation of eNOS activation and production of NOS are a ripe target for mathematical modeling, we are not aware of such a model when associated with nicotine effects as mentioned above in smokers and non-smokers