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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

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Acquired by Japan Tobacco International in 2007, Sobranie is one of the fastest-growing brands in JTIs portfolio, having enjoyed unstoppable growth since 2012 in all markets where it is present, according to Ahern