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Briefly, cells (1 10 4 cells/well) were seeded in 48-well plates in EBM-2 medium (Lonza) with EGM-2 supplements

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The average 5-year survival rate has remained practically unchanged over the last 40 years: at 7583% for patients with localized HNSCC, 4565% for those whose cancer spread to regional lymph nodes, and only 3340% for those with distant metastasis 2

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