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Downstream analysis for the microbiota The microbiota analysis was conducted as previously published 11 and as deposited at the corresponding GitHub repository: The analysis was performed using R (version 4.3.1), with the packages phyloseq (version 1.44), microbiome (version 1.22) and vegan (version 2.6-8)

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We further analyzed the concentration-dependent increasing curves to compare the pharmacological action characteristics of different nicotine salts

Factors such as pre-existing mental health conditions, personal circumstances, and genetic predispositions can influence an individual's vulnerability to depression after quitting smoking