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The medium fabric density balances breathability with enough weight to survive the colder seasons

Nuclei were stained with DAPI (Sigma)

Another writer of the day, Sarah Loring Bailey, wrote in her history of Andover: There have been forty professors [in the history of the seminary], but their wives and daughters, six women, have published books which have had a circulation of at least a million copies. No doubt, Bailey vastly under-represented the circulation of those womens works (because one of them was Harriet Beecher Stowe), but she rightly emphasizes the wide readership of the reform works penned by the women of Andover

The nebulizer gas (nitrogen) flow was 6.0 bar