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Maintenance The primary issue with these machines is tobacco build-up on the bottom of the cutter (TOP calls it a tamper) and on the top/front of the chamber (viewed through the nozzle) Even a light build-up on the cutter can (it depends on how tight the clearances are to start with) noticeably affect the 'feel' of the machine (without tobacco there is noticeable resistance when the cutter is 'cutting')

The Badgers characteristic "Salt & Peber" colour scheme is clearly represented in our choice of the mouthpiece and the dark brown/black staining of the bowl and shank mimics the Badgers preferred surroundings

The slides were incubated with a biotinylated secondary antibody for 30 min, and ABC solution for 30 min, washing with PBS between each process

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