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Following incubation, the membranes were washed, and densitometric analysis was conducted via ImageJ software (NIH) to quantify the intensity of the protein bands

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18,19 Analysis of NDSHS data from 2001 to 2010 found that the overall rate of hardcore smoking (defined as the number of people who smoked more than 15 cigarettes a day, had not made an attempt to quit in the last 12 months, and did not plan to quit) remained stable over the 10 years, but varied by socioeconomic status (SES)

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