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In addition, the number of Treg cells and the expression of IL-10 and Foxp3 were lower in the lungs of mice exposed to cigarette smoke than in the control group

There were differences in aerosol VG and PG concentrations between the e-cigarette variants consistent with the specified e-liquid compositions shown in Table 1

Cigarette smoke exposure HBE cells were exposed to mainstream smoke from 3R4F University of Kentucky reference cigarettes (Lexington, KY) using a VC-10 Smoking Robot (Vitrocell, Waldkirch, Germany) in accordance with ISO 3308 standards (puff duration 2 s, hold time 0 s, exhaust time 8 s, puff volume 35 mL, frequency 60 s, 6 puffs per cigarette)

Unknown effects Health and addiction experts are less enthusiastic about the normalisation of a product whose effects remain relatively unknown